Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips

Figure 1

Specificity analysis of the partial sequence of 23S rRNA gene and verification of the recombinant plasmid. (a) The partial sequence was analyzed using Nucleotide BLAST with genome of C. burnetii excluded and the BLAST hits were shown; (b) the recombinant plasmid was digested with BamH I and EcoR I, and the products were analyzed using agarose gel electrophoresis; M, DNA marker; P, digested products.

(a)

(b)