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BioMed Research International
Volume 2018 (2018), Article ID 6439481, 8 pages
https://doi.org/10.1155/2018/6439481
Research Article

Successful Formulation and Application of Plant Growth-Promoting Kosakonia radicincitans in Maize Cultivation

1Leibniz Institute of Vegetable and Ornamental Crops, Grossbeeren, Germany
2Institute for National and International Plant Health, Julius Kühn-Institute, Federal Research Centre for Cultivated Plants, Braunschweig, Germany
3Algorithms in Bioinformatics, Center for Bioinformatics, University of Tuebingen, Tuebingen, Germany
4ABiTEP GmbH, Berlin, Germany

Correspondence should be addressed to Matthias Becker; ed.vezgi@saihttam.rekceb

Received 15 November 2017; Revised 29 January 2018; Accepted 18 February 2018; Published 28 March 2018

Academic Editor: Paulina E. De los Santos

Copyright © 2018 Beatrice Berger et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The global market for biosupplements is expected to grow by 14 percent between 2014 and 2019 as a consequence of the proven benefits of biosupplements on crop yields, soil fertility, and fertilizer efficiency. One important segment of biosupplements is plant growth-promoting bacteria (PGPB). Although many potential PGPB have been discovered, suitable biotechnological processing and shelf-life stability of the bacteria are challenges to overcome for their successful use as biosupplements. Here, the plant growth-promoting Gram-negative strain Kosakonia radicincitans DSM (family Enterobacteriaceae) was biotechnologically processed and applied in the field. Solid or liquid formulations of K. radicincitans were diluted in water and sprayed on young maize plants (Zea mays L.). Shelf-life stability tests of formulated bacteria were performed under 4°C and −20°C storage conditions. In parallel, the bacterial formulations were tested at three different farm level field plots characterized by different soil properties. Maize yield was recorded at harvest time, and both formulations increased maize yields in silage as well as grain maize, underlining their positive impact on different agricultural systems. Our results demonstrate that bacteria of the family Enterobacteriaceae, although incapable of forming spores, can be processed to successful biosupplements.