Purification and Characterization of Antibodies in Single-Chain Format against the E6 Oncoprotein of Human Papillomavirus Type 16

<div>Expression and purification of anti-16E6 scFvs. (a) Schematic representation of the scFvI7 and scFvI7nuc proteins. Light chain (<i>V</i><sub>L</sub>), polylinker, and heavy chain (<i>V</i><sub>H</sub>) domains are shown. N-terminal His-tag and C-terminal V5 tag allow purification and immunodetection, respectively. The SV40 nuclear localization signal and Myc-tag present in scFvI7nuc are indicated. (b) Purification of anti-16E6 scFvs by Ni-NTA chromatography. SDS-PAGE analysis and CBB staining of aliquots of the scFvI7nuc eluates obtained at pH 5.9 (lanes 1–4) and pH 4.5 (lanes 5–9). (c) SDS-PAGE analysis and CBB staining of scFvI7nuc and scFvI7 after purification and refolding. M: protein markers.</div>

BioMed Research International

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Figure 1

Figure 1: Purification and Characterization of Antibodies in Single-Chain Format against the E6 Oncoprotein of Human Papillomavirus Type 16 