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BioMed Research International
Volume 2018, Article ID 6716547, 11 pages
https://doi.org/10.1155/2018/6716547
Research Article

Angelica gigas Nakai Has Synergetic Effects on Doxorubicin-Induced Apoptosis

1Department of Biological Sciences, Konkuk University, Neungdong-ro 120, Gwangjin-gu, Seoul 05029, Republic of Korea
2Korea Institute of Science and Technology, Gangneung, Gangwondo 25451, Republic of Korea
3Department of Convergence Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea
4Korea Hemp Institute, Konkuk University, Konkuk University, Neungdong-ro 120, Gwangjin-gu, Seoul 05029, Republic of Korea
5Department of Advanced Technology Fusion, Konkuk University, Neungdong-ro 120, Gwangjin-gu, Seoul 05029, Republic of Korea

Correspondence should be addressed to Kyungho Lee; rk.ca.kuknok@ohgnuyk and Ye Sun Han; rk.ca.kuknok@nahsy

Received 7 March 2018; Revised 11 July 2018; Accepted 24 July 2018; Published 1 August 2018

Academic Editor: Claudio Tabolacci

Copyright © 2018 Yong-Joon Jeon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Natural products are valuable sources for drug discovery because they have a wide variety of useful chemical components and biological properties. A quick reevaluation of the potential therapeutic properties of established natural products was made possible by the recent development of the methodology and improvement in the accuracy of an automated high-throughput screening system. In this study, we screened natural product libraries to detect compounds with anticancer effects using HeLa cells. Of the 420 plant extracts screened, the extract of Angelica gigas Nakai (AGN) was the most effective in reducing cell viability of HeLa cells. Markers of apoptosis, such as exposure of phosphatidylserine and cleavage of caspase-7 and PARP, were increased by treatment with the AGN extract. Treatment of the AGN extract increased expression of PKR as well as ATF4 and CHOP, the unfolded protein response genes. In addition, cotreatment of doxorubicin and the AGN extract significantly increased doxorubicin-induced apoptosis in HeLa cells. Decursin and decursinol angelate, which were known to have anticancer effects, were the main components of the AGN extract. These results suggest that the extract of AGN containing, decursin and decursinol angelate, increases doxorubicin susceptibility.