(a)
(b)
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Figure 4: Administration of the AGN extract activated the ATF4-CHOP pathway in doxorubicin-treated HeLa cells. (a) The cells were cotreated with 1 μM doxorubicin and 1 μg/ml AGN extract for the indicated time periods and immunoblot analyses were performed using the indicated antibodies. (b, c) The mRNA level of ATF4 and CHOP were determined by real-time quantitative PCR. Statistical significance of the difference as calculated by Student’s t-test is with p<0.01 or p<0.001. (d) The cells were cotreated with 1 μM doxorubicin and 1-2 μg/ml AGN extract for 24 h as indicated and immunoblot analyses were performed using the indicated antibodies.