Effects of Astaxanthin on Miniature Pig Sperm Cryopreservation

Figure 1

Flow cytometric analysis of sperm labeled with Yo-Pro-1/HE fluorescence in control and AXT treated groups. V1 region represents viable sperm with a low intracellular O₂ radical, and V2 region represents viable sperm with a high intracellular O₂ radical, and the upper quadrant Dead and Moribund regions represent dead sperm, in the process of programmed cell death of sperm. Yo-Pro-1/HE fluorescence: measure O₂ radical of sperm. Yo-Pro-1: FL1 Log; HE: FL3 Log.