Effects of Astaxanthin on Miniature Pig Sperm Cryopreservation

Figure 3

Microscopic view of sperm stained with Yo-Pro-1/HE (a) or PI/H₂DCFDA (b) fluorescence ( FL, Thermo Fisher Scientific). The images indicate apoptotic cells (green), whereas dead nonapoptotic cells are red in color. Four populations of sperm were obtained: light green or clear (live cells), dark green (apoptotic cells), red (dead/necrotic cells), and red and green (dead cells).

(a)

(b)