Establishment of a Gene Detection System for Hotspot Mutations of Hearing Loss

Table 6

values obtained from the different platforms.

Mutation Site	LightCycler 96		LightCycler 480		Bio-Rad CFX-96
	wt (°C)	mu (°C)	wt (°C)	mu (°C)	wt (°C)	mu (°C)
rs74315319	72	65	72	65	70.5	64
rs111033313	60	54	58	52.5	58	52
rs121908362	67	61	66	60	65	59
rs267606617	67	61	66	61	65	59
rs267606619	67	59	66	58	64.5	57
rs750188782	71	58	69	55	68	54
rs80338943	77	72	76.5	71.5	75	71
rs1110333204	63	69	61.5	67.5	60.5	66.5
rs80338939	72	67	72	67.5	70.5	66.5