Colour Vignetting Correction for Microscopy Image Mosaics Used for Quantitative Analyses

Figure 1

Vignetting correction versus blending. (a) Mosaic obtained by stitching together two partially overlapped images, without performing any tonal correction. On the right, the intensity plot profile of the dashed-blue line highlighted in the mosaic on the left. (b) Same mosaic as (a) but obtained by vignetting correction. The intensity profile became flat. (c) Same mosaic as (a) but obtained by minimizing the discontinuities in the stitching zones through blending. Globally, intensity discontinuities still remain in the mosaic as shown by the plot profile.

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(b)

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