Colour Vignetting Correction for Microscopy Image Mosaics Used for Quantitative Analyses

Figure 2

Colour mosaics of cells and tissues. (a, c) Mosaics obtained by stitching two partly overlapping images, without performing any tonal correction. On the right, a high-magnification detail representing a region across the stitching seam (highlighted with red arrowheads in the magnified details). The images in (a) do not have saturated pixels, while those in (c) have pixels saturated in the red channel. (b, d) Same mosaics as (a) and (c), respectively, but obtained by vignetting correcting the images according to (2). Seams in the stitching zones are still visible in (d).

(a)

(b)

(c)

(d)