Inflammatory Human Umbilical Cord-Derived Mesenchymal Stem Cells Promote Stem Cell-Like Characteristics of Cancer Cells in an IL-1β-Dependent Manner

Figure 2

Effects of UC-MSCs on proliferation and apoptosis of cancer cells. (a-b) UC-MSC conditioned medium (MSC-CM) 1 : 1 mixed with fresh basic medium was supplemented with 10% FBS and used for culturing. CCK8 assay was performed in MDA-MB-231 (a) or IGROV1 cells (b) at the indicated time points, and OD value was measured at 450 nm. (c-d) MDA-MB-231 (c) or IGROV1 cells (d) were indirectly cocultured with UC-MSCs and harvested at the indicated time points, and the percentage of Ki67 positive cells was shown here. ; ns stands for nonsignificance. (e-f) Annexin V-PI staining was performed in MDA-MB-231 (e) or IGROV1 cells (f) cocultured with UC-MSCs, and apoptotic cells were analyzed by FACS. Representative dot plots and statistical results were shown here. ; ns stands for nonsignificance.

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