Behavior of Smooth Muscle Cells under Hypoxic Conditions: Possible Implications on the Varicose Vein Endothelium

Table 1

RT-qPCR primer sequences and binding temperatures (Temp).

GENE	SEQUENCE Fwd (5′→3′)	SEQUENCE Rev (5′→3′)	Temp
HIF-1	ACGTGTTATCTGTCGCTTTGAG	ATCGTCTGGCTGCTGTAATAATG	59°C
EGLN3	GATGCTGAAGAAAGGGC	CTGGCAAAGAGAGTATCTG	60°C
TGF-1	GCGTGCTAATGGTGGAAAC	CGGAGCTCTTGATGTGTTGAAGA	60°C
VEGF	ATGACGAGGGCCTGGAGTGTG	CCTATGTGCTGGCCTTGGTGAG	60°C
eNOS	AAG AGG AAG GAG TCC AGT AAC ACA GA	ACG AGC AAA GGC GCA GAA	60°C
Tie-2	TGCCCAGATATTGGTGTCCT	CTCATAAAGCGTGGTATTCACGTA	60°C
GAPDH	ATGACGAGGGCCTGGAGTGTG	CCTATGTGCTGGCCTTGGTGAG	60°C