Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2018 (2018), Article ID 7174561, 10 pages
https://doi.org/10.1155/2018/7174561
Research Article

Differentiation Model Establishment and Differentiation-Related Protein Screening in Primary Cultured Human Sebocytes

1Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China
2Department of Nephrology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China
3Department of Gastroenterology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China

Correspondence should be addressed to Yi-Ming Fan; moc.361@3691nafmy and Ge-Shi; moc.361@67honid

Received 28 December 2017; Accepted 6 March 2018; Published 5 April 2018

Academic Editor: Ichiro Kurokawa

Copyright © 2018 Man-Feng Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sebocyte differentiation is a continuous process, but its potential molecular mechanism remains unclear. We aimed to establish a novel sebocyte differentiation model using human primary sebocytes and to identify the expression profiles of differentiation-associated proteins. Primary human sebocytes were cultured on Sebomed medium supplemented with 2% serum for 7 days. Flow cytometry showed that S phase cells were decreased time-dependently, while G1 and subG1 (apoptosis) phase cells increased under serum starvation. Transmission electron microscopy and Oil Red O staining revealed a gradual increase of intracellular lipid accumulation. Expression of proliferation marker was diminished, while expression of differentiation, apoptosis, and lipogenic markers elevated gradually during 7-day culture. iTRAQ analysis identified 3582 expressed proteins in this differentiation model. Compared with day 0, number of differentially expressed proteins was 132, 54, 321, and 96 at days 1, 3, 5, and 7, respectively. Two overexpressed proteins (S100 calcium binding protein P and ferredoxin reductase) and 2 downexpressed proteins (adenosine deaminase and keratin 10) were further confirmed by Western blot and immunohistochemistry.