Antioxidant, Hepatoprotective, and Antidepression Effects of Rumex tingitanus Extracts and Identification of a Novel Bioactive Compound

Table 1

The IC₅₀ and AAI values of DPPH test and β-carotene-linoleic acid assay and IC_0.5 values of reducing power assay of R. tingitanus leaves extracts.

Extract	DPPH		β-Carotene	Reducing power
	IC50 (µg/ml)	AAI	IC50 (µg/ml)	IC0.5 (µg/ml)
Rt EtOH-H2O	78.1 ± 1.5	0.51	451 ± 9	106 ± 3.1
Rt EtOAcF	11.7 ± 0.2	3.39	320 ± 16	41 ± 2
Rt HexF	193.8 ± 7.9	0.26	>2000	410 ± 24.6
Rt WF	81.4 ± 4	0.49	>2000	133 ± 7.9
Luteolin	12.7 ± 0.2	3.12	19 ± 1	nt
4′-p-acetylcoumaroyl luteolin	20.7 ± 0.6	1.92	nt	nt
BHT	86.5 ± 1.7	0.46	5.01 ± 0.15	41 ± 2.1
Ascorbic acid	3.7 ± 0.2	10.61	9.60 ± 0.01	nt

BHT and ascorbic acid were used as standard; IC₅₀ (µg/mL): values corresponding to the extract amount required to scavenge 50% of radicals present in the reaction mixture; IC_0.5: extract or compound concentration providing absorbance of 0.5; AAI: antioxidant activity index; nt: not tested.