BioMed Research International

Research Article

Screening Potential Probiotic Characteristics of Lactobacillus brevis Strains In Vitro and Intervention Effect on Type I Diabetes In Vivo

Table 2

Significance, means, and standard errors (SE) of factors affecting the growth rate of KLDS 1.0727 and KLDS 1.0373 strains.
SOV/ExperimentsGrowth rateBile tolerance SIJ with different pH aerobically SIJ with different pH anaerobically Antibiotic resistanceAdhesion to Caco2 cell
HighLowHighLowHighLowHighLowHighLowHighLow
Strain(S1)(S2)(S1)(S2)(S1)(S2)(S2)(S1)(S2)(S1)(S2)(S1)
Mean±SE of strains1.24±0.1.15±0.210.79 ±0.76±0.080.36±0.36±0.070.51±0.49±0.100.45±0.44±0.06245 ±244.67±33.31
Time270270240240243--
Mean±SE of time1.97±0.22±0.031.40±1.10±0.010.56±0.14±0.011.05±0.14±0.011.11±0.05±0.003--
Concentration of different parameters --020202Cont.Amp.30
Mean±SE-- ±0.150.52±0.071.00±0.12±0.011.39±0.15±0.010.80 ±0.05±0.003381.67±137.50±6.26
R2 99.9999.9699.9899.8899.9972.36
CV 0.672.112.646.711.8330.48
In the six experiments, growth rate: the effect of two strains KLDS 1.0727 (S1) and KLDS 1.0373 (S2), and five levels of times 0, 3, 9, 27, and 33h on growth rate and pH; bile tolerance: the effect of two strains, five levels of times, and four levels of bile salts concentration Cont, 0.3, 1, and 2% on growth rate and pH; simulated intestinal juice (SIJ) with different pH aerobically: the effect of two strains, five levels of times 0, 3, 6, 9, and 24, and simulated intestinal juice with different four levels of pH Cont, 2, 3, and 7 on growth rate; simulated intestinal juice(SIJ) with different pH anaerobically: the effect of two strains, five levels of times 0, 3, 6, 9, and 24, and simulated intestinal juice with different four levels of pH Cont, 2, 3, and 7; antibiotic resistance: the effect of two strains, five levels of times, and types of antibiotics Cont, streptomycin, ampicillin, chloramphenicol, erythromycin, gentamycin, and kanamycin on growth rate and pH; adhesion with Caco2 cell: the effect of two strains, four levels of dilution of 2 strains Cont, 3, 4, and 5 adhesion of Caco 2 cell. Significance: a, P 0,01; b: P 0,001; c: P 0,0001; d: nonsignificant. R2: determination coefficient, and CV: coefficient of variation. SOV: source of variance.