Genetic Polymorphism in the RYR1 C6487T Is Associated with Severity of Hypospadias in Chinese Han Children

Figure 1

Electrophoresis diagram of PCR products. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to test the RYR1 C6487T polymorphism. The electrophoresis results of PCR amplification of target gene fragment were exhibited. The wild-type homozygote CC without restriction site yielded a fragment of 253 bp in length, heterozygote CT yielded three fragments of 253 bp, 108 bp, and 145 bp in length, and mutant homozygote TT yielded two fragments of 108 bp and 145 bp in length. PCR products performed SNP sequencing after electrophoresis, recovery, and purification. M: DNA marker; 1, wild-type homozygote; 2, heterozygote; 3, mutant homozygote; PCR, polymerase chain reaction.