Mutational Analysis of Oncogenic AKT1 Gene Associated with Breast Cancer Risk in the High Altitude Ecuadorian Mestizo Population

Table 1

Primer sequences, annealing temperatures, and genetic variations.

Polymorphisms	Primer sequences	Annealing temperatures	Location	Fragment (bp)
rs121434592; E17K	FW 5’- GGC CAA GGG GAT ACT TAC GC -3’	60	Exon 4	198
	RV 5’- AGG GTC TGA CGG GTA GAG TG -3’
rs11555432; L357P	FW 5’- CCT TCT TGA GCA GCC CTG AA -3’	56.5	Exon 12	142
	RV 5’- TAC GAG ATG ATG TGC GGT CG -3’
rs12881616; E319G	FW 5’- CAG AGA GGA CAC AGC ATT GCG -3’	60.5	Exon 10	186
	RV 5’- ACA AGG ACG GGC ACA TTA AGA -3’
rs11555431; P388T	FW 5’- CAA GGA CAT CAA GCT TTG GCT -3’	61	Exon 11	171
	RV 5’- AAG TCC TTG CTT TCA GGG CT -3’
rs3803304	FW 5’- CAA GGA CAT CAA GCT TTG GCT -3’	61	Intron 13	171
	RV 5’- AAG TCC TTG CTT TCA GGG CT -3’
rs2494732	FW 5’- CAA GGA CAT CAA GCT TTG GCT -3’	61	Intron 13	171
	RV 5’- AAG TCC TTG CTT TCA GGG CT -3’

bp, base pairs.