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BioMed Research International
Volume 2018 (2018), Article ID 7497314, 8 pages
https://doi.org/10.1155/2018/7497314
Research Article

The CD40 rs1883832 Polymorphism Affects Sepsis Susceptibility and sCD40L Levels

1Translational Medicine Center of Sepsis, Department of Pathophysiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China
2Department of Critical Care Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China
3Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, China

Correspondence should be addressed to Xian-Zhong Xiao; moc.621@2102zxxxdnz and Ming-Shi Yang; moc.621@smyxdnz

Received 30 August 2017; Revised 9 January 2018; Accepted 14 February 2018; Published 26 March 2018

Academic Editor: Ruxana Sadikot

Copyright © 2018 Zuo-Liang Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sepsis is a severe and progressive disease characterized by systemic inflammatory response syndrome (SIRS). CD40 serves as a vital link between immune response and inflammation. This study was designed to investigate the potential association between a functional single-nucleotide polymorphism (SNP) of CD40 (rs1883832) and susceptibility to sepsis. We first performed a case-control study to explore the relationship between the CD40 rs1883832 polymorphism and sepsis. CD40 mRNA expression and protein expression were determined by real-time PCR and western blotting, respectively, in peripheral blood mononuclear cells (PBMCs) from sepsis patients and healthy controls. The plasma sCD40L levels in the two groups were measured by ELISA. The results showed that the frequencies of the TT genotype and the CD40 rs1883832 T allele were significantly higher in sepsis patients than in healthy controls. Plasma sCD40L levels were also significantly increased in sepsis patients. In addition, TT genotype carriers among sepsis patients displayed the highest CD40 expression at both the mRNA and protein levels, accompanied by the highest plasma sCD40L concentrations. In conclusion, the CD40 rs1883832 T allele acts as a risk factor for increased susceptibility to sepsis and may be involved in the process of sepsis through regulation of CD40 expression and plasma sCD40L levels.