The Carotenogenic Dunaliella salina CCAP 19/20 Produces Enhanced Levels of Carotenoid under Specific Nutrients Limitation

Figure 6

Modification of protein synthesis in Dunaliella salina CCAP 19/20 during stress growth conditions of macro- and micronutrients depletion. Protein samples (2 μg), from healthy green cells actively grown for 5 days in complete medium (modified ATCC 1174DA) (GP_control), in stress medium (complete medium devoid of KNO₃) (Control_stress), and in two other stress media (complete medium devoid of Cu or CuMn in addition to KNO₃ depletion), were resolved by SDS-PAGE (10%) and visualized by colloidal Coomassie dye G-250 staining. To determine the molecular weights of prominent polypeptides either missing, appearing new, or at enhanced levels, a broad-range proteins molecular weight mixture (New England Biolabs) were run simultaneously.