| Repeat | ITS71 () | ITS197 () | ITS296 () | AcaP | AceP | AcaP | AceP | AcaP | AceP |
| First () | 86.1 ± 0.1°C | 88.0 ± 0.1°C | 85.1 ± 0.1°C | 85.9 ± 0.1°C | 85.0 ± 0.1°C | 84.0 ± 0.1°C | Second () | 86.1 ± 0.2°C | 88.1 ± 0.1°C | 85.1 ± 0.1°C | 85.9 ± 0.1°C | 85.0 ± 0.1°C | 84.0 ± 0.2°C | Third () | 86.1 ± 0.1°C | 87.9 ± 0.1°C | 85.1 ± 0.2°C | 85.9 ± 0.1°C | 85.1 ± 0.1°C | 84.1 ± 0.1°C | Average () | 86.10°C | 88.00°C | 85.10°C | 85.90°C | 85.03°C | 84.03°C | CV | 0.15% | 0.09% | 0.14% | 0.18% | 0.07% | 0.13% |
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