2³ Full Factorial Model for Particle Size Optimization of Methotrexate Loaded Chitosan Nanocarriers: A Design of Experiments (DoE) Approach

Table 1

Coded and actual values of formulation variables.

Factor; (Variable)		Low Level	Mid-Level	High Level
	Coded; Value	-1	0	+1
Amount of Chitosan X1 (mg/ml)		1	1.5	2
Amount of TPP X2 (mg/ml)		0.8	0.9	1.0
Amount of MTX X3 (mg)		30	45	60

mid-level was followed for center point formulations.