LincRNA-p21 Inhibits Cell Viability and Promotes Cell Apoptosis in Parkinson’s Disease through Activating -Synuclein Expression

Figure 3

-synuclein was the target gene of miR-1277-5p. (a) The sequence of wide-type α-synuclein or mutated α-synuclein was inserted into the 3′ UTR of renilla luciferase (hRluc). (B) The luciferase activity was suppressed significantly after transfection of miR-1277-5p mimics in α-synuclein WT group. The luciferase activity was stimulated markedly after transfection of miR-1277-5p inhibitor in α-synuclein WT group. However, there was no difference in α-synuclein Mut group. (c, d, e) The α-synuclein mRNA and protein expression levels were measured after transfected with miR-1277-5p mimics or inhibitor. (f, g) The rescue effects of α-synuclein on cell viability and apoptosis after overexpression of miR-1277-5p in SH-SY5Y cells treated with MPP⁺ were presented. All data are shown as the mean ± SD (P < 0.05, P < 0.01, P < 0.001); bar, SD.

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