DREAM Is Involved in the Genesis of Inflammation-Induced Prolabour Mediators in Human Myometrial and Amnion Cells

Figure 2

Effect of siDREAM on the proinflammatory cytokine IL-6 in primary myometrial cells. Primary myometrial cells were transfected with 50 nM siCONT or 50 nM siDREAM for 48 h and then treated with (a, b) 100 pg/mL IL-1β, (c, d) 250 ng/ml fsl-1, (e, f) 1 μg/ml flagellin, or (g, h) 5 μg/ml poly(I:C) for an additional 24 h (patients). (a, c, e, g) IL-6 mRNA expression was analysed by qRT-PCR. (b, d, f, h) The incubation media was assayed for concentration of IL-6 by ELISA. For all data, fold change was calculated relative to IL-1β-, fsl-1, and flagellin- or poly(I:C)-stimulated siCONT-transfected cells. Data are displayed as mean ± SEM. versus IL-1β-stimulated siCONT-transfected cells; versus fsl-1-stimulated siCONT-transfected cells; versus flagellin-stimulated siCONT-transfected cells; versus poly(I:C)-stimulated siCONT-transfected cells (one-way ANOVA).

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