Ischemic Preconditioning Promotes Autophagy and Alleviates Renal Ischemia/Reperfusion Injury

Figure 1

Hypoxia/reoxygenation (H/R) injury protocol. Control: cells were cultured constantly under normal conditions. H/R: cells received 15 h of oxygen and glucose deprivation (OGD) and 30 min, 1 h, 2 h, 6 h, or 12 h of reoxygenation in normal complete medium. Hypoxic preconditioning (HPC): transient OGD for 6 h and subsequent reoxygenation for 2 h in normal complete medium before prolonged H/R injury.