Anti-Inflammatory Effect of Geniposide on Osteoarthritis by Suppressing the Activation of p38 MAPK Signaling Pathway

Figure 2

Effects of geniposide on inflammatory mediators and p38 MAPKs signaling pathway in OA chondrocytes in vitro. (a) qRT-PCR analysis of IL-1β, TNF-α, and MMP-13 gene expression in OA chondrocytes incubated with geniposide and SB203580 as well as the combination of these two drugs, respectively. The data of control were considered as 1. (b) Western blotting of IL-1β, TNF-α, MMP-13, p-p38 (phosphorylation of p38), and t-p38 (total p38) protein expression among four groups. (c) Quantification of western blotting. The expression level of TNF-α, IL-1β, MMP-13, and NO in the supernatants of the OA chondrocytes (d–g) treated with geniposide and SB203580 as well as the combination of these two drugs, respectively. Data is given as the mean ± SD; and .

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