Basic Fibroblast Growth Factor Fused with Tandem Collagen-Binding Domains from<i> Clostridium histolyticum </i>Collagenase ColG Increases Bone Formation

<div>SDS-polyacrylamide gel showing the molecular masses of the various collagen-binding bFGF proteins. GST-fusion proteins of collagen-binding bFGF (CB-bFGFs) were produced using pGEX-4T vectors. The proteins were purified by glutathione affinity chromatography, and the GST moiety was cleaved off using thrombin proteinase. CB-bFGFs were isolated using a Heparin-Sepharose column and the proteins (1 <i>μ</i>g each) were analyzed by SDS-polyacrylamide gel electrophoresis (15% gel). M, molecular mass marker; 1, bFGF-s3 (calculated mass, 30,920.37); 2, bFGF-s2b-s3 (calculated mass, 41,516.07); 3, bFGF-s3b (calculated mass, 30,925.34); and 4, bFGF-s3a-s3b (calculated mass, 44,193.58). Numbers on the left indicate molecular mass (kDa) according to the molecular marker.</div>

BioMed Research International

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Figure 2

Figure 2: Basic Fibroblast Growth Factor Fused with Tandem Collagen-Binding Domains from<i> Clostridium histolyticum </i>Collagenase ColG Increases Bone Formation 

Figure 2 | Basic Fibroblast Growth Factor Fused with Tandem Collagen-Binding Domains from Clostridium histolyticum Collagenase ColG Increases Bone Formation 