Basic Fibroblast Growth Factor Fused with Tandem Collagen-Binding Domains from Clostridium histolyticum Collagenase ColG Increases Bone Formation

Table 1

Binding affinity of various collagen anchors.

Collagen anchor	(×10^−5 M)
s3	75.2 ± 0.41
s2b-s3	44.5 ± 0.55
s3b	4.54 ± 0.15
s3a-s3b	4.46 ± 0.45

Anchor proteins were dissolved in HBS-Ca buffer at concentrations ranging from 1 × 10⁻⁷ M to 3 × 10⁻⁴ M. Binding to the collagenous peptide, H-Gly-Pro-Arg-Gly-(Pro-Hyp-Gly)₁₂-NH₂, was measured by surface plasmon resonance. Data were directly fitted to the equation described in the Materials and Methods by the least squares method to calculate the apparent dissociation constant and uncertainty values.