Genome-Wide Investigation and Expression Profiling of HD-Zip Transcription Factors in Foxtail Millet (Setaria italica L.)

Figure 2

Phylogenetic relationships, structure, and motif analysis of the 47 predicted foxtail millet and 25 green foxtail HD-Zip proteins. (a) The tree was generated with the MEGA 7.0 program using the full-length amino acid sequence by the NJ method. Bootstrap values are indicated at the nodes. Each subfamily shown in the tree is marked with differently colored backgrounds. (b) Exon/intron structure of sihdz genes. Exons and introns are presented by green boxes and black lines, and untranslated regions (UTRs) are indicated by blue boxes. (c) Distribution of conserved motifs in foxtail millet HD-Zip family members. All motifs were identified using the MEME website. Differently colored boxes represent different motifs with their names on the right, and colored boxes are ordered manually according to the results of MEME analysis. The length of the box does not present the actual motif size in the protein.