Deregulation of Hexokinase II Is Associated with Glycolysis, Autophagy, and the Epithelial-Mesenchymal Transition in Tongue Squamous Cell Carcinoma under Hypoxia

Figure 2

Effects of hypoxia on the glycolysis of TSCC cells. (a) Glucose consumption in TSCC cells significantly increased under hypoxia for different periods of time. (b) Lactate production in TSCC cells dramatically increased under hypoxia. (c) Hypoxia induced higher expression of HIF-1α, Glut-1, and HKII in all three cell types. (d) The HIF-1α levels were estimated as a ratio with the levels of GAPDH for all three cell lines. (e) The quantitative Glut-1 data was shown as a ratio with the levels of GAPDH in TSCC cells. (f) The quantitative HKII analysis was presented after normalization to GAPDH in TSCC cells. All results are presented as mean ± SD obtained from three independent experiments. , , and .

(a)

(b)

(c)

(d)

(e)

(f)