Autophagy Suppresses Invasiveness of Endometrial Cells through Reduction of Fascin-1

Figure 3

The effects of treatment with the autophagy activator rapamycin on the formation of filopodia and cellular invasiveness. The tagged filopodial marker protein FLAG-fascin was overexpressed to visualize the filopodia (red) and DAPI staining was used to visualize the nuclei (gray). (a) Control treatment (buffer). (b) Rapamycin treatment. (c) Results of the statistical analysis of the relative length of filopodia. The results were normalized to the control group, which was set to 1. (d) Scratch assay analysis of the cells’ invasiveness. The results were normalized to the control group, which was set to 100%. p<0.01 compared with the control.

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