The Involvement of Arginase and Nitric Oxide Synthase in Breast Cancer Development: Arginase and NO Synthase as Therapeutic Targets in Cancer

Figure 4

Arginase activity (▲) and protein quantity (■) curves after CM-cellulose chromatography of human serum arginase (stage II, , ). (a) Healthy, (b) breast cancer, and (c) chemotherapy. The enzyme obtained after gel chromatography (9, 11, and 13 mg protein, resp., for healthy individuals, breast cancer patients, and patients after chemotherapy) to a CM-cellulose column (1 × 18 cm) equilibrated with 5 mM Tris-HCI, pH 7.5, eluted with a KCl concentration gradient. Fractions (4 ml) were collected at a flow rate of 24 ml/h.

(a)

(b)

(c)