Research Article
Comprehensive Validation of Snapback Primer-Based Melting Curve Analysis to Detect Nucleotide Variation in the Codon 12 and 13 of KRAS Gene
Table 1
Summary of samples with simultaneous KRAS mutation in TCGA and MSK-IMPACT cohort.
| | Cancer Study | Sample ID | Tumor Tissue | AA Change | Start Pos | End Pos | Reference | Variation | Mutant Allele Freq |
| | TCGA | TCGA-2J-AAB1-01 | Pancreas | G12D | Chr12:25398284 | Chr12:25398284 | C | T | NA | | G12R | Chr12:25398285 | Chr12:25398285 | C | G | 0.13 | | TCGA | TCGA-2J-AABH-01 | Pancreas | G12V | Chr12:25398284 | Chr12:25398284 | C | A | 0.19 | | G12S | Chr12:25398285 | Chr12:25398285 | C | T | 0.19 | | TCGA | TCGA-FB-A78T-01 | Pancreas | G12A | Chr12:25398284 | Chr12:25398284 | C | G | 0.14 | | G13C | Chr12:25398282 | Chr12:25398282 | C | A | 0.15 | | TCGA | TCGA-YB-A89D-01 | Pancreas | G12D | Chr12:25398284 | Chr12:25398284 | C | T | NA | | G12R | Chr12:25398285 | Chr12:25398285 | C | G | 0.17 | | MSK-IMPACT | P-0002475-T01-IM3 | Metastatic Tumor | G12D | Chr12:25398284 | Chr12:25398284 | C | T | 0.31 | | G13D | Chr12:25398281 | Chr12:25398281 | C | T | 0.38 | | MSK-IMPACT | P-0006073-T01-IM5 | Metastatic Tumor | G12D | Chr12:25398284 | Chr12:25398284 | C | T | 0.05 | | G12C | Chr12:25398285 | Chr12:25398285 | C | A | 0.13 | | MSK-IMPACT | P-0006634-T01-IM5 | Metastatic Tumor | G13D | Chr12:25398281 | Chr12:25398281 | C | T | 0.02 | | G12I | Chr12:25398284 | Chr12:25398285 | CC | AT | 0.15 | | MSK-IMPACT | P-0008464-T02-IM5 | Metastatic Tumor | G12V | Chr12:25398284 | Chr12:25398284 | C | A | 0.03 | | G12R | Chr12:25398285 | Chr12:25398285 | C | G | 0.20 | | MSK-IMPACT | P-0009837-T01-IM5 | Metastatic Tumor | G12F | Chr12:25398284 | Chr12:25398285 | CC | AA | 0.05 | | G12C | Chr12:25398285 | Chr12:25398285 | C | A | 0.13 | | MSK-IMPACT | P-0010007-T01-IM5 | Metastatic Tumor | G12V | Chr12:25398284 | Chr12:25398284 | C | A | 0.05 | | G12A | Chr12:25398284 | Chr12:25398284 | C | G | 0.17 | | MSK-IMPACT | P-0011012-T01-IM5 | Metastatic Tumor | G12C | Chr12:25398285 | Chr12:25398285 | C | A | 0.03 | | G12S | Chr12:25398285 | Chr12:25398285 | C | T | 0.06 |
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