Comprehensive Validation of Snapback Primer-Based Melting Curve Analysis to Detect Nucleotide Variation in the Codon 12 and 13 of KRAS Gene

Table 3

Intra- and interassay precision of the snapback primer system for KRAS mutation detection.

	Intra-assay		Inter-assay
	G12D	G13D	G12D	G13D
Mean (Melting temperature)	67.6	68.5	67.5	68.7
Standard Deviation	0.16	0.15	0.29	0.31
Coefficient of variance (%)	0.23	0.22	0.43	0.45