Pinocembrin Protects Blood-Brain Barrier Function and Expands the Therapeutic Time Window for Tissue-Type Plasminogen Activator Treatment in a Rat Thromboembolic Stroke Model

Figure 5

PCB decreased t-PA-induced MMP-2 and MMP-9 activation and TJs (occludin and claudin-5) loss in thromboembolic stroke. ((a) and (b)) the MMP-2 and MMP-9 protein levels in the contralateral (Con) and ipsilateral (Ips) brain tissue in t-PA and PCB + t-PA-treated rats and the quantitative analysis result. STD, the standard of MMP-2 or MMP-9. ((c) and (d)) Western blot analysis of occludin and claudin-5 in cell membrane with different treatments. Rats received t-PA (1 mg/kg) and PCB (10 mg/kg) and tissues were analyzed 4 hr after varied hours of thromboembolic stroke in different groups ( = 3; < 0.05, versus nonischemic, paired -test).

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