Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model

Figure 1

Analysis of model establishment and plasmid transfection. Representative ultrasound images (a–d) are longitudinal and transverse sections of the arteries in the HFD and OD groups (the white arrows indicate the lumen of the arteries). Representative pathological images ((e) and (f)) show the average thickness of the arterial intima in the HFD and OD groups. Representative fluorescence and western blot images (g-j) of arteries transfected by plasmids. The white arrows (g) indicate the intima transfected by the two plasmids (pc-salusin-α and si-salusin-α) with the EGFP reporter gene in the transfection groups, and the image in (h) indicates the arterial wall transfected by no plasmids. The western blot image (i) shows the EGFP gene expression in three different groups, which were Salusin-alpha gene overexpression (pc-salusin-α), salusin-alpha gene interference (si-salusin-α), and no plasmids transfection (NO-plasmid) group. Similarly, the salusin-alpha gene expression in the three groups was displayed by (j). The data are representative of three independent experiments .