Identification of Differentially Expressed Genes in Porcine Ovaries at Proestrus and Estrus Stages Using RNA-Seq Technique

Table 1

RNA-Seq data statistics and annotation information results.

Samples	Estrus 1	Estrus 2	Estrus 3	Proestrus 1	Proestrus 2	Proestrus 3
Raw reads number	43,537,742	44,980,724	46,933,870	43,251,414	42,674,930	43,682,540
Raw bases	6,518,495,731	6,737,054,480	7,029,003,258	6,477,569,804	6,391,485,035	6,542,005,180
Clean reads number	39,553,500	40,781,504	42,823,178	38,910,588	38,772,168	39,721,342
Clean bases	5,921,488,722	6,107,904,887	6,413,011,132	5,827,088,794	5,806,734,458	5,948,483,660
Clean rate (%)	90.85	90.66	91.24	89.96	90.85	90.93
Q20 (%)	96.80	96.55	96.73	96.54	96.79	96.83
Q30 (%)	91.59	91.00	91.41	91.02	91.59	91.72
Mapped reads	30,184,209	32,430,870	34,341,651	31,166,703	31,287,495	32,149,872
Uniquely mapped reads	25,723,220	29,028,295	29,898,722	28,575,851	27,616,922	28,678,605
Multiple mapped reads	4,460,989	3,402,575	4,442,929	2,590,852	3,670,573	3,471,267
Transcript Number	24,217	38,128	28,636	37,748	31,381	30,867
Exon total length (bp)	36,281,115	83,700,310	49,116,462	89,558,585	64,040,781	57,382,487
Average transcript length (bp)	1,498	2,195	1,715	2,373	2,041	1,859
Max transcript length (bp)	8,370	19,865	11,037	22,330	32,100	13,205
Min transcript length (bp)	112	153	126	150	146	150
N50 length (bp, without intron)	1,986	3,061	2,326	3,361	2,800	2,525