Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein

<div>PVX-derived vectors for the expression of iLOV and the infection of N. benthamiana. (a) The iLOV coding sequence was added either as a direct fusion to the 5′-end of the PVX coat protein (CP) gene, with a (G4S)3 linker, or as a fusion to the FMDV 2A sequence. The target genes are depicted as part of the complete viral genome. (b–f) Symptoms and detection of fluorescence in N. benthamiana plants inoculated with hybrid PVX. (b) Noninfected N. benthamiana plant and plants infected with (c) wild-type PVX, (d) pPVX-iLOV-CP, (e) pPVX-iLOV-2A-CP, and (f) pPVX-iLOV-G4S-CP were displayed either under UV light or under normal light 10–34 days after inoculation (dpi) depending on the progress of infection.</div>

BioMed Research International

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Figure 1

Figure 1: Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein

Figure 1 | Systemic Infection of Nicotiana benthamiana with Potato virus X Nanoparticles Presenting a Fluorescent iLOV Polypeptide Fused Directly to the Coat Protein 