Lactobacillus plantarum with Functional Properties: An Approach to Increase Safety and Shelf-Life of Fermented Foods

Table 1

Molecular methods/techniques used for identification of Lb. plantarum strains.

Molecular method	Primer used	Primer sequence (5′-3′)	Identified Lb. plantarum strain	Reference
PCR	LbPI1 (forward) and LbPI2 (reverse)	CCG TTT ATG CGG AAC ACC TA and TCG GGA TTA CCA AAC ATC AC	Lb. plantarum ATCC 8014	Quere et al. [18]
RAPD-PCR	16S rRNA-based primer, P32	TAC CAC TAC AAT GGA TG	Lb. plantarum ATCC 14917	Elegado et al. [19]
RAPD-PCR	16S rRNA-based primer, A27F	AGC GGA TCA CTT CAC ACA GGA CTA CGG CTA CCT TGT TAC GA	Lb. plantarum YW11	Wang et al. [20]
RAPD-PCR	UB16S-F and UB16S-R	AGA GTT TGA TCC TGG CTC AG and ACG GCT ACC TTG TTA CGA CT	Lb. plantarum NTMI05 and NTMI20	Imran et al. [21]
16S rDNA sequencing	Universal primer, SSU	TGC CAG CAG CCG CGG TA and GAC GGG CGG TGT GTA CAA	Lb. plantarum B128, B134, B143, B149, B166, B174	Mahmoudi et al. [22]
16S rDNA sequencing	8f and 1512r	CAC GGA TCC AGA CTT TGA T(C/T)(A/C) TGG CTC AG and; GTG AAG CTT ACG G(C/T)T AGC TTG TTA CGA CTT	Lb. plantarum MBSa4	Barbosa et al. [23]
RAPD and PFGE	OPA5 and OPA20	AAT CGG GCT G and GTT GCG TCC	Lb. plantarum ATCC 8014 and Lb. plantarum SD1S612	Adesulu-Dahunsi et al. [24]
16S rDNA sequencing	27F and 1492R	AGA GTT TGA TCC TGG CTC AG and; TAC GGY TAC CTT GTT ACG ACT T	Lb. plantarum KX881772 and Lb. plantarum KX881779	Abushelaibi et al. [25]
ITS-PCR	16SF-R2 and 23SR-R10	AGA GTT TGA TCC TGG CTC AG and; AAG GAG GTG ATC CAG CCG CA	Lb. plantarum GA106, FU137, NRRLB-14768, DSM10667, JCM1558, DK0–22, OB123, OF101, YO175	Adesulu-Dahunsi et al. [24]

RAPD-PCR: random amplified polymorphic DNA-polymerase chain reaction; PCR: polymerase chain reaction; PFGE: pulsed-field gel electrophoresis; ITS-PCR: 16S–23S rRNA gene intergenic transcribed spacer PCR amplification. enzyme (ApaI and SfiI).