Research Article
T-Synthase Deficiency Enhances Oncogenic Features in Human Colorectal Cancer Cells via Activation of Epithelial-Mesenchymal Transition
Figure 2
Forced deletion of T-synthase in HCT116 cells via the CRISP/CAS9 method. (a) One pair of single guide RNA (sgRNA) was designed to specifically target C1GALT1 that encodes T-synthase. (2) Western blot analysis was performed to confirm deletion of T-synthase in cells. (3) Tn antigen expression, indicative of aberrant O-glycosylation, was determined by flow cytometry in cells.
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