MiR-92a Inhibits the Progress of Osteosarcoma Cells and Increases the Cisplatin Sensitivity by Targeting Notch1

Figure 4

MiR-92a directly targets Notch1 in osteosarcoma cell. (a) A predicted schematic of the seed region of miR-92a in the 3’-UTR of Notch1 and the mutated 3’-UTR of Notch1 by bioinformatics. (b) Relative luciferase activity of the wild-type or mutant pGL3-Notch1-3′-UTR plasmid in OG63 and HOS cells cotransfected with miR-92a mimic or negative control. (c) The protein expression of Notch1 was detected by western blotting. (d) The mRNA expression of Notch1 was detected by qRT-PCR. Data represent mean ± SD. P < 0.05; P < 0.01.

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