BioMed Research International / 2019 / Article / Fig 4

Research Article

Transcription Factor IRF4 Dysfunction Affects the Immunosuppressive Function of Treg Cells in Patients with Primary Immune Thrombocytopenia

Figure 4

After RNA silencing of IRF4 gene of Treg cells of healthy controls, the regulation function of Tregs damaged. (a) Real-time PCR was used to detect the effectiveness of IRF4mRNA silencing. Negative: negative control. Mock: the calibration set. Representative figure of cells micrographs (×200) of two fragments (H342 and H422) after RNA silencing of IRF4 gene of Tregs. (b) Representative figure of cell micrographs (×200) of two fragments (H342 and H422) after RNA silencing of Tregs, cocultured with CD4+CD25-Teffs to a ratio of 1:8. (c) Representative dot plots of Th17 cells in NC, H342, and H422 groups after RNA silencing of Tregs, cocultured with CD4+CD25-T cells. (All CD4+T cells were sorted with microbeads.) (d) The percentage of Th17 cells in CD4+T cells of three cocultured groups. (e) IL-17 ELISA test for three groups’ coculture supernatant. (f) The expression of IL-17mRNA of three groups after being cocultured. H342: IRF4-Homo-342, H422: IRF4-Homo-422; NC: normal control. p < 0. 05; p < 0. 01; p < 0. 001.
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