Research Article

Combined Activation of Guanylate Cyclase and Cyclic AMP in Lung Fibroblasts as a Novel Therapeutic Concept for Lung Fibrosis

Figure 3

Concentration and time dependent effect of TGF-β1 treatment on collagen type-I de novo synthesis and deposition in the absence and presence of sGC and cAMP activators. (a) Inhibition of TGF-β1 by LY2157299 (10−6 M), or preincubation (30 min) with BAY 41-2272 (10−6 M) or forskolin (10−6 M) significantly reduced transcription of the collagen type-I gene at 24 hours. (b) Concentration dependent induction of collagen type I deposition by TGF-β1. (c) Inhibition of collagen type I deposition by increasing concentration of BAY 41-2272 and forskolin in TGF-β1 stimulated fibroblasts at 24 hours. Bars represent mean ±SEM of triplicate experiments.
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