Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma

Figure 2

Methylation rates of patients with breast cancer: Figure shows methylation rates in patients with breast cancer. Methylation rates were found between 33 and 58% for PAX5 gene and 49 and 55% for TMPRSS2 gene, respectively. Four of 10 breast cancer primary tumor tissues were found as methylated for both PAX5 and TMPRSS2 (bc22, bc27, bc21, bc15). Methylation was not seen in 2D and 3D tissue culture systems for samples of bc22, bc24, bc27, bc21, bc16, bc38, bc10. Methylation in PAX5 gene was not seen in any types of tissues of bc12 tumors. 2D: two-dimensional tissue culture, 3D: three-dimensional tissue culture, TD: primary tumor tissue, and BC: breast cancer.