Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma

Table 4

Methylation rates (%) of three genes in patients with breast cancer (2D: two-dimensional tissue culture, 3D: three-dimensional tissue culture, T: primary tumor tissue, and BC: breast cancer).

Specimens		Methylation Rates ()
Specimen 1		bc22 - 2D	bc22 - 3D	bc22 – T
Genes	PAX5	0	0	51
	TMPRSS2	0	0	50
	SBDS	0	0	0
Specimen 2		bc24 - 2D	bc24 - 3D	bc24 – T
Genes	PAX5	0	0	0
	TMPRSS2	0	0	51
	SBDS	0	0	0
Specimen 3		bc26 - 2D	bc26 - 3D	bc26 – T
Genes	PAX5	0	44	52
	TMPRSS2	0	0	0
	SBDS	0	0	0
Specimen 4		bc27 - 2D	bc27 - 3D	bc27 – T
Genes	PAX5	0	0	51
	TMPRSS2	0	0	49
	SBDS	0	0	0
Specimen 5		bc21 - 2D	bc21 - 3D	bc21 – T
Genes	PAX5	0	0	58
	TMPRSS2	0	0	49
	SBDS	0	0	0
Specimen 6		bc12 - 2D	bc12 - 3D	bc12 – T
Genes	PAX5	0	0	0
	TMPRSS2	49	50	50
	SBDS	0	0	0
Specimen 7		bc15 - 2D	bc15 - 3D	bc15 – T
Genes	PAX5	0	0	52
	TMPRSS2	49	55	51
	SBDS	0	0	0
Specimen 8		bc16 - 2D	bc16 - 3D	bc16 – T
Genes	PAX5	0	0	43
	TMPRSS2	0	0	0
	SBDS	0	0	0
Specimen 9		bc38 - 2D	bc38 - 3D	bc38 – T
Genes	PAX5	0	0	33
	TMPRSS2	0	0	0
	SBDS	0	0	0
Specimen 10		bc10 - 2D	bc10 - 3D	bc10 - T
Genes	PAX5	0	0	0
	TMPRSS2	0	0	0
	SBDS	0	0	0