Methylation Changes of Primary Tumors, Monolayer, and Spheroid Tissue Culture Environments in Malignant Melanoma and Breast Carcinoma

Table 6

Methylation rates of patients with malignant melanoma (2D: two-dimensional tissue culture, 3D: three-dimensional tissue culture, T: primary tumor tissue, and mg: malignant melanoma).

Specimens	Methylation Rates () in
	2 Dimensional Cell Culture ()	3 Dimensional Cell Culture ()	Primer Tumor Tissue ()
Specimen 1
PAX5	52	51	50
TMPRSS2	0	0	37
SBDS	0	0	0
Specimen 2
PAX5	35	35	31
TMPRSS2	0	0	0
SBDS	0	0	0
Specimen 3
PAX5	22	24	42
TMPRSS2	45	46	50
SBDS	0	0	38
Specimen 4
PAX5	68	65	55
TMPRSS2	47	45	39
SBDS	0	0	0
Specimen 5
PAX5	50	48	42
TMPRSS2	45	42	41
SBDS	0	0	0
Specimen 6
PAX5	50	52	50
TMPRSS2	51	52	48
SBDS	0	0	0