Research Article

Long Noncoding RNA MALAT1 Acts as a Competing Endogenous RNA to Regulate TGF-β2 Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells by a MicroRNA-26a-Dependent Mechanism

Figure 5

Knockdown of MALAT1 inhibits Smad4, a target of miR-26a. (a) Smad4 protein levels in primary HLECs were detected by Western blot analysis. The primary HLECs were treated with TGF-β2 (5 ng/ml) for 48 h before incubation with MALAT1 siRNAs for 24 h or anti-miR-26a for 6 h. (b) The expression levels of Smad4 mRNA were detected by qRT-PCR. The primary HLECs were treated with TGF-β2 (5 ng/ml) for 48 h before incubation with MALAT1 siRNAs for 24 h or anti-miR-26a for 6 h. P < 0.05 compared with normal or TGF-β2 group. #P < 0.05 compared with TGF-β2+MALAT1 siRNAs group. ##P < 0.05 compared with TGF-β2 group and TGF-β2+MALAT1 siRNAs group. All of data are presented as the mean± SE of six independent experiments.

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