Expression Profiling of Exosomal miRNAs Derived from the Peripheral Blood of Kidney Recipients with DGF Using High-Throughput Sequencing

Figure 1

Identification of exosomes through nanoparticle tracking analysis, transmission electron microscopy, and Western blot. (a) Concentration and size of exosomes in control (a) and DGF (b) groups were analyzed by the nanoparticle tracking analysis. (c) Exosomes isolated from the peripheral blood of recipients one week after kidney transplantation have a saucer-like shape that was limited by a lipid bilayer. Green arrowheads point to exosomes. Scale bar = 100 nm. (d) Exosomes are positive for CD63 and CD9, as determined through Western blot analysis. β-Actin was used as a positive control.

(a)

(b)

(c)

(d)