(a)
(b)

Figure 2: Length and category distribution of sequenced exosomal miRNAs in the peripheral blood of kidney recipients. (a) Category distribution of sequenced exosomal miRNAs. Reads with length 18–26 nt were chosen for mapping. Length distribution of sequenced miRNAs is shown. The nucleotide (nt) lengths of cloned miRNAs are shown on x-axis; the number of total reads by deep sequencing is shown on y-axis. (b) Category distribution of sequenced exosomal miRNAs. Gp1a: reads were mapped to miRNAs/pre-miRNAs of specific species in miRBase and the pre-miRNAs were further mapped to genome and EST. gp2a: reads were mapped to miRNAs/pre-miRNAs of selected species in miRBase and the mapped pre-miRNAs were not further mapped to genome, but the reads (and of course the miRNAs of the pre-miRNAs) were mapped to genome. The extended genome sequences from the genome loci may form hairpins. gp2b: reads were mapped to miRNAs/pre-miRNAs of selected species in miRBase and the mapped pre-miRNAs were not further mapped to genome, but the reads (and of course the miRNAs of the pre-miRNAs) were mapped to genome. The extended genome sequences from the genome loci may not form hairpins. gp3: reads were mapped to miRNAs/pre-miRNAs of selected species in miRBase and the mapped pre-miRNAs were not further mapped to genome, and the reads were not mapped to genome either. But the reads were mapped to the miRNAs (Matures). gp4: reads were not mapped to pre-miRNAs of selected species in miRBase. But the reads were mapped to genome and the extended genome sequences from genome may form hairpins.