The lengths of LM and LAM are modulated during growth in minimal medium. (a) Glycolipid fractions of the mycobacterial cell wall of M. smegmatis, in the stationary phase of growth in Middlebrook 7H9 broth (7H9+Gly) or minimal medium (MM) with or without glycerol and/or cholesterol supplementation (7H9+Gly, MM+Gly, MM+Gly+Chol, MM+Chol, and MM groups), were separated by electrophoresis in 4-20% Tris-Tricine SDS-PAGE and stained for glycans to visualize LM and LAM. (b) Densitometric analysis of stained LM and LAM in 4-20% Tris-Tricine SDS-PAGE was performed using the ImageJ 1.48v software. The staining of LM and LAM is a representative result of three independent experiments, showing only the relevant segment of the SDS-PAGE. MW: molecular weight standard.