Muscle Atrophy Marker Expression Differs between Rotary Cell Culture System and Animal Studies

Figure 2

Alginate encapsulated undifferentiated (a) and differentiated cells (b) imaged at 2x magnification, scale bar = 1000 μm. Interior morphology of encapsulated differentiated cells in a 30 μm section of an alginate bead (c) compared to a standard T25 (d), scale bar = 100 μm. Nuclei are stained blue with Hoechst 33342 ((c) & (d)). Mitochondria are stained red with MitoTracker CMX-Ros ((d) only).

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(b)

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(d)